Abstract
Coenzyme Q10 (CoQ10) is a potent endogenous antioxidant, which has high medical and industry value. In this study, improved CoQ10 production in Brevundimonas diminuta was reported after optimizing medium constituents, carbon sources, natural precursor supplementation, and fermentation approaches. Batch and fed-batch fermentations were conducted with glucose, sucrose, and glycerol as carbon sources and tomato/tobacco extracts as natural precursors. Glucose was found to be the best carbon source for both biomass production and CoQ10 accumulation. Fed-batch fermentation using alternating glucose feeding enhanced the CoQ10 yield by 22% in fed-batch culture compared to batch. Intracellular CoQ10 was isolated, purified by silica gel chromatography, and quantitated by HPLC. Kinetic analysis also indicated high substrate consumption and growth-related CoQ10 formation, indicating the successfulness of the optimized fed-batch approach.
Key Words: Coenzyme Q10, Brevundimonas diminuta, Fed-batch fermentation, Natural precursors, Microbial bioprocess optimization
Citation: Srivastava J et al., (2025) Enhanced Microbial Production of Coenzyme Q10 Using Fed-Batch Fermentation and Natural Precursors. Journal of Current Engineering and Allied Science.9(4):1-8